St Mary's Hospital 1 article published in JoVE Medicine Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples Ryan S Thwaites1, Hannah C Jarvis1, Nehmat Singh1, Akhilesh Jha1, Andy Pritchard2, Hailing Fan1, Tanushree Tunstall1, Joan Nanan1, Simon Nadel2, Onn Min Kon2, Peter J Openshaw1, Trevor T Hansel1 1 This manuscript describes the use of nasal and bronchial absorption techniques, specifically using synthetic absorptive matrices (SAM) to sample the mucosal lining fluid (MLF) of the upper and lower airway. These methods provide better standardization and tolerability than existing respiratory sampling techniques.