University of Patras 3 articles published in JoVE Neuroscience The "Brain Milking" Method for the Isolation of Neural Stem Cells and Oligodendrocyte Progenitor Cells from Live Rats Dimitrios Dimitrakopoulos1, Chrisitna Dimitriou1, Freyja McClenahan2, Robin J. M. Franklin2,3, Ilias Kazanis1 1Laboratory of Developmental Biology, Department of Biology, University of Patras, 2Wellcome Trust-MRC Cambridge Stem Cell Institute, University of Cambridge, 3Altos Labs, Cambridge Institute of Science A method for the isolation of neural stem cells and oligodendrocyte progenitor cells from the brains of live rats is presented here in experimental detail. It allows multiple collections of these cells from the same animals without compromising their well-being. Bioengineering A GMP-Compliant Procedure for the Generation of Gene-Modified T cells Nikolaos Savvopoulos1, Klearchos Stampolitis1, Georgios Alexandropoulos1, Dionysia Kefala1, Memnon Lysandrou1, Vassiliki Zacharioudaki1, Nikos Tsolakos2, Alexandros Spyridonidis1 1Institute of Cell Therapy, Bone Marrow Transplantation Lab, Department of Medicine, University of Patras, 2Protatonce Ltd, Demokritos Science Park This protocol outlines the process of transducing primary human T cells with a gene of interest, ensuring compatibility with Good Manufacturing Practice (GMP) standards. Biology Murine Dermal Lymphatic Endothelial Cell Isolation Racheal Grace Akwii*1, Margarita Lamprou*2, Maria Georgomanoli3, Andriana Plevriti2, Antonia Marazioti4, Athanasia Mouzaki5, George Mattheolabakis6, Constantinos M. Mikelis1,2 1Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, 2Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, 3Division of Flow Cytometry and Division of MDx & Life Sciences, SB BioAnalytica, 4Basic Sciences Laboratory, Department of Physiotherapy, University of Peloponnese, 5Division of Hematology, Department of Internal Medicine, Medical School, University of Patras, 6School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana at Monroe This paper describes a method for magnetic bead-based isolation of murine endothelial cells from dermal lymphatic capillaries. The isolated lymphatic endothelial cells can be used for downstream in vitro experiments and protein expression analysis.
