University of Peloponnese 1 article published in JoVE Biology Murine Dermal Lymphatic Endothelial Cell Isolation Racheal Grace Akwii*1, Margarita Lamprou*2, Maria Georgomanoli3, Andriana Plevriti2, Antonia Marazioti4, Athanasia Mouzaki5, George Mattheolabakis6, Constantinos M. Mikelis1,2 1Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, 2Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, 3Division of Flow Cytometry and Division of MDx & Life Sciences, SB BioAnalytica, 4Basic Sciences Laboratory, Department of Physiotherapy, University of Peloponnese, 5Division of Hematology, Department of Internal Medicine, Medical School, University of Patras, 6School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana at Monroe This paper describes a method for magnetic bead-based isolation of murine endothelial cells from dermal lymphatic capillaries. The isolated lymphatic endothelial cells can be used for downstream in vitro experiments and protein expression analysis.
