University of Galway 1 article published in JoVE Biology Polycarbonate Ultracentrifuge Tube Re-Use in Proteomic Analyses of Extracellular Vesicles Rachel M. E. Cahalane*1,2, Mandy E. Turner*1, Cassandra L. Clift1, Mark C. Blaser1, Gabrielle Bogut1, Sydney Levy1, Taku Kasai1, Tom A. P. Driedonks3,4, Esther N. M. Nolte-‘t Hoen4, Masanori Aikawa1,5, Sasha A. Singh1,5, Elena Aikawa1,5 1Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School A detailed protocol is provided for cleaning and re-using polycarbonate ultracentrifuge tubes to perform extracellular vesicle isolation suitable for proteomics experiments.
