Ruijsink Dynamic Engineering 1 article published in JoVE Behavior An Automated Method to Determine the Performance of Drosophila in Response to Temperature Changes in Space and Time Andrea Soto-Padilla1,2, Rick Ruijsink3, Mark Span4, Hedderik van Rijn*4, Jean-Christophe Billeter*1 1Groningen Institute for Evolutionary Life Sciences, University of Groningen, 2Department of Cell Biology, University of Groningen, University Medical Center Groningen, 3Ruijsink Dynamic Engineering, 4Department of Psychology, University of Groningen Here we present a protocol to automatically determine the locomotor performance of Drosophila at changing temperatures using a programmable temperature-controlled arena that produces fast and accurate temperature changes in time and space.