NOVA Medical School 1 article published in JoVE Medicine Functional and Physiological Methods of Evaluating Median Nerve Regeneration in the Rat Diogo Casal*1,2, Eduarda Mota-Silva*3, Inês Iria4,5, Diogo Pais1, Ana Farinho6, Sara Alves7, Cláudia Pen7, Luís Mascarenhas-Lemos7, José Ferreira-Silva7, Mário Ferraz-Oliveira7, Valentina Vassilenko3, Paula A. Videira4,5, João Goyri-O'Neill1 1Anatomy Department, NOVA Medical School, 2Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central, 3Physics Department, Faculty of Sciences and Technology, LIBPhys, 4Glycoimmunology, CEDOC, NOVA Medical School, 5UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, 6Tissue Repair Unit, CEDOC, NOVA Medical School, 7Pathology Department, Centro Hospitalar de Lisboa Central Presented is a protocol to produce different types of median nerve (MN) lesions and repair in the rat. Additionally, the protocol shows how to evaluate the functional recovery of the nerve using several noninvasive behavioral tests and physiological measurements.