Great Ormond Street Hospital View Institution's Website 2 articles published in JoVE Developmental Biology The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System Enrica Bertin1, Martina Piccoli1, Chiara Franzin1, Andras Nagy2, Maria Mileikovsky2, Paolo De Coppi3, Michela Pozzobon1 1Stem Cell and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Citta della Speranza, 2Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 3Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital In this study, we generate induced pluripotent stem cells from mouse amniotic fluid cells, using a non-viral-based transposon system. Neuroscience Electrophysiological Measurements and Analysis of Nociception in Human Infants L. Fabrizi*1, A. Worley*2, D. Patten1, S. Holdridge1, L. Cornelissen1, J. Meek3, S. Boyd2, R. Slater1,4 1Neuroscience, Physiology and Pharmacology, University College London, 2Department of Clinical Neurophysiology, Great Ormond Street Hospital, 3Elizabeth Garrett Anderson Obstetric Hospital, University College Hospital, 4Nuffield Department of Anaesthetics, University of Oxford The assessment and treatment of pain in infants is difficult because infants cannot verbally report their experience. In this video we describe quantitative electrophysiological methods and analysis techniques that can be used to measure the response to noxious events from the infant nervous system.