University of Saskatchewan View Institution's Website 7 articles published in JoVE Immunology and Infection Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury Jessica Andrea Brocos Duda1, Manpreet Kaur1, Gurpreet K. Aulakh1 1Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan Combined ozone and bacterial endotoxin exposed mice show wide-spread cell death, including that of neutrophils. We observed cellular adaptations such as disruption of cytoskeletal lamellipodia, increased cellular expression of complex V ATP synthase subunit β and angiostatin in broncho-alveolar lavage, suppression of the lung immune response and delayed neutrophil recruitment. Genetics Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq) Melissa B. Palmer1,2, Yejun Wang3, Aaron P. White1,2 1Vaccine and Infectious Disease Organization-International Vaccine Centre, 2Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, 3Department of Cell Biology and Genetics, Shenzhen University Health Science Center Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) is a method used to establish interactions between transcription factors and the genomic sequences they control. This protocol outlines techniques for performing ChIP-seq with bacterial biofilms, using Salmonella enterica serovar Typhimurium bacterial biofilm as an example. Developmental Biology Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans Kunal Baxi1, Carlos E. de Carvalho1 1Department of Biology, University of Saskatchewan A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA) Environment Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas Youn Young Shim1,2,3, Rana Mustafa1, Jianheng Shen1, Kornsulee Ratanapariyanuch1, Martin J. T. Reaney1,2,3 1Department of Plant Sciences, University of Saskatchewan, 2Prairie Tide Chemicals Inc., 3Guangdong Saskatchewan Oilseed Joint Laboratory (GUSTO), Department of Food Science and Engineering, Jinan University Aquafaba is a viscous juice from canned chickpea that, when stirred vigorously, produces a relatively stable white froth or foam. The primary research goal is to identify the components of aquafaba that contribute viscosifying/thickening properties using nuclear magnetic resonance (NMR), ultrafiltration, electrophoresis, and peptide mass fingerprinting. Neuroscience The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster Soeren Diegelmann1, Annika Jansen1, Shreyas Jois1,2, Katharina Kastenholz1, Laura Velo Escarcena1, Nicole Strudthoff1, Henrike Scholz1 1Institute of Zoology, Albertus-Magnus University of Cologne, 2Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan The CApillary FEeder (CAFE) assay is a simple, budget-friendly, highly reliable method for investigating mechanisms underlying food intake. Used with the highly versatile genetic model organism Drosophila melanogaster, it provides a powerful means of gaining new insights into regulatory mechanisms of food intake. Immunology and Infection Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres Tim J. Dumonceaux1, Jennifer R. Town1, Janet E. Hill2, Bonnie L. Chaban2, Sean M. Hemmingsen3 1Saskatoon Research Centre, Agriculture and Agri-Food Canada, 2Department of Veterinary Microbiology, University of Saskatchewan, 3Plant Biotechnology Institute, National Research Council of Canada We describe a multiplex method for the detection of microorganisms within a sample using oligonucleotide-coupled fluorescent beads. Amplicon from all organisms within a sample is hybridized to a panel of probe-coupled beads. A Luminex or Bio-Plex instrument is used to query each bead for bead type and hybridization signal. Immunology and Infection Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy Najia Xu1, Xi Lei1, Lixin Liu1 1Department of Pharmacology, University of Saskatchewan We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.