Health Canada View Institution's Website 2 articles published in JoVE Chemistry HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues Nikolai L. Chepelev1, Dean A. Kennedy1, Remi Gagné1, Taryn White1, Alexandra S. Long1, Carole L. Yauk1, Paul A. White1 1Environmental Health Science and Research Bureau, Health Canada The goal of this protocol is the detection of the DNA oxidation marker, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) by HPLC-ED, in DNA from cultured cells or animal tissues. Immunology and Infection Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays Caroline Gravel*1, Changgui Li*2, Junzhi Wang2, Anwar M Hashem1,3,4, Bozena Jaentschke1, Gary Van Domselaar5, Runtao He5, Xuguang Li1,3 1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB, Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 4Microbiology Department, Faculty of Medicine, King Abdulaziz University, 5National Microbiology Laboratory, Public Health Agency of Canada A simple slot blot method was developed for the quantification of influenza viral hemagglutinin and neuraminidase using universal antibodies targeting their most conserved sequences identified through bioinformatics analyses. This innovative approach may provide a useful alternative to quantitative determination of all viral hemagglutinin and neuraminidase.