Claremont McKenna College 2 articles published in JoVE Biology Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks Nathan Felcher1, Daisy Achiriloaie1, Brian Lee2, Ryan McGorty3, Janet Sheung1,2,4 1W.M. Keck Science Department, Scripps College, 2W.M. Keck Science Department, Claremont McKenna College, 3Department of Physics and Biophysics, University of San Diego, 4W.M. Keck Science Department, Pitzer College This protocol describes in detail how to build a single-objective, light-sheet fluorescence microscope and its usage for visualizing cytoskeleton networks. Neuroscience Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections Yuu Ohno1, Riley Murphy2, Matthew Choi3, Weijun Ou4, Rachita K. Sumbria4,5 1Henry E. Riggs School of Applied Life Sciences, Keck Graduate Institute, 2Crean College of Health and Behavioral Sciences, Chapman University, 3Keck Science Department, Claremont McKenna College, 4Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy, Chapman University, 5Department of Neurology, University of California, Irvine The present protocol describes and compares the procedure to perform a full-region or sub-region of interest analysis of sagittal mouse brain sections to quantify amyloid-beta load in the APP/PS1 transgenic mouse model of Alzheimer's disease.
