Harvard School of Dental Medicine 3 articles published in JoVE Biology Transverse Fracture of the Mouse Femur with Stabilizing Pin Emily R. Moore1, Marina Feigenson2, David E. Maridas1 1Department of Developmental Biology, Harvard School of Dental Medicine, 2Keros Therapeutics This protocol describes a method to perform fractures on adult mice and monitor the healing process. Biology Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity Andrew J. Collins*1,2, Pallavi P. Murugkar*1,2, Floyd E. Dewhirst1,2 1The Forsyth Institute, 2Harvard School of Dental Medicine We demonstrate a method for isolating difficult-to-grow members of the novel bacterial phylum, Saccharibacteria, by filtering dental plaque and co-culturing with host bacteria. Medicine Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation Ruiye Bi1,2, Yi Fan2,3, En Luo2,4, Quan Yuan2,4, Michael Mannstadt1 1Endocrine Unit, Massachusetts General Hospital, Harvard Medical School, 2West China School of Stomatology, Sichuan University, 3Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, 4State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University Mice with acquired hypoparathyroidism would be useful for studying novel drug therapies for hypoparathyroidism. Two procedures to create such mice are demonstrated. The GFP-PTX mouse is generated by surgical parathyroidectomy guided by green fluorescing parathyroid glands. A second, non-surgical approach is based on parathyroid-specific expression of the diphtheria toxin receptor.