Harvard John A. Paulson School of Engineering and Applied Sciences 1 article published in JoVE Bioengineering Modeling Healthy and Dysbiotic Vaginal Microenvironments in a Human Vagina-on-a-Chip Aakanksha Gulati1, Alicia Jorgenson1, Abidemi Junaid1, Donald E. Ingber1,2,3 1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2 This article describes a protocol for creating a microfluidic vagina-on-a-chip (Vagina Chip) culture device that enables the study of human host interactions with a living vaginal microbiome under microaerophilic conditions. This chip can be used as a tool to investigate vaginal diseases as well as to develop and test potential therapeutic countermeasures.