Thermo Fisher Scientific 1 article published in JoVE Biochemistry Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow Alan Tin Lun Lam1, Premkumar Jayaraman2, Abigail Becker3, Ryan Lim2, Kim Leng Teo1, Jacqueline Ng2, Steve Oh1 1Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A STAR), 2Thermo Fisher Scientific, 3Thermo Fisher Scientific Here, we present a protocol to harvest adherent cells from multi-layered flasks in a closed semi-automated manner using a counterflow centrifugation system. This protocol can be applied for harvesting both adherent and suspension cells from other cell expansion platforms with few modifications to the existing steps.