xyZfish 1 article published in JoVE Biology Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish Hans Maaswinkel1, Liqun Zhu1, Wei Weng1 1R&D, xyZfish The use of a 3D automatic video system that can track individual and groups of zebrafish is described. As application example we explore the effects of the NMDA-receptor antagonist MK-801 on shoals of zebrafish.