St. John Fisher College 1 article published in JoVE Bioengineering Rapid Fabrication of Custom Microfluidic Devices for Research and Educational Applications Megan Levis*1,2, Fernando Ontiveros*3, Jonathan Juan1, Anthony Kavanagh1, Jeremiah J. Zartman1,2 1Department of Chemical and Biomolecular Engineering, University of Notre Dame, 2Bioengineering Graduate Program, University of Notre Dame, 3Biology Department, St. John Fisher College Here we present a protocol to design and fabricate custom microfluidic devices with minimal financial and time investment. The aim is to facilitate the adoption of microfluidic technologies in biomedical research laboratories and educational settings.