Saint Joseph’s University 1 article published in JoVE Medicine Boldness, Aggression, and Shoaling Assays for Zebrafish Behavioral Syndromes Gregory P. Way1,2, Maura Southwell1, Scott P. McRobert1 1Department of Biology, Saint Joseph’s University, 2Genomics and Computational Biology, University of Pennsylvania This manuscript describes the setup, implementation, and analysis of boldness, aggression, and shoaling in zebrafish and testing for the presence of a behavioral syndrome. A standardized approach for behavioral quantification will allow for easier comparison across studies. Modifications to this protocol are possible as each assay can be run individually.