Methodist Hospital, Houston View Institution's Website 6 articles published in JoVE Medicine Time-Resolved, Dynamic Computed Tomography Angiography for Characterization of Aortic Endoleaks and Treatment Guidance via 2D-3D Fusion-Imaging Marton Berczeli1,2, Ponraj Chinnadurai1,3, Su Min Chang4, Alan B. Lumsden1 1Department of Cardiovascular Surgery, Houston Methodist Hospital, 2Department of Vascular and Endovascular Surgery, Semmelweis University, 3Advanced Therapies, Siemens Medical Solutions USA Inc., 4Department of Cardiology, Houston Methodist Hospital Dynamic computed tomography angiography (CTA) imaging provides additional diagnostic value in characterizing aortic endoleaks. This protocol describes a qualitative and quantitative approach using time-attenuation curve analysis to characterize endoleaks. The technique of integrating dynamic CTA imaging with fluoroscopy using 2D-3D image fusion is illustrated for better image guidance during treatment. Cancer Research Acellular and Cellular Lung Model to Study Tumor Metastasis Dhruva K. Mishra1, Min P. Kim1,2 1Department of Surgery, Houston Methodist Hospital Research Institute, 2Division of Thoracic Surgery, Department of Surgery, Weill Cornell Medical College, Houston Methodist Hospital Here, we present a protocol for an ex vivo lung cancer model that mimics the steps of tumor progression and helps to isolate a primary tumor, circulating tumor cells, and metastatic lesions. Biology Functional Imaging of Brown Fat in Mice with 18F-FDG micro-PET/CT Xukui Wang1, Laurie J. Minze2, Zheng-Zheng Shi1 1Department of Translational Imaging, The Methodist Hospital Research Institute, Houston, 2Diabetes Research Center, The Methodist Hospital Research Institute, Houston A method of functional imaging of mouse brown adipose tissue (BAT) is described in which cold-stimulated uptake of 18F-Fluorodeoxyglucose (FDG) in BAT is non-invasively assessed with a standardized micro-PET/CT protocol. This method is robust and sensitive to detect differences in BAT activities in mouse models. Bioengineering Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery Jia Fan1,2, James W. Gallagher1, Hung-Jen Wu1, Matthew G. Landry1, Jason Sakamoto1, Mauro Ferrari1, Ye Hu1 1Department of Nanomedicine, The Methodist Hospital Research Institute, 2CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology We developed a technology based on mesoporous silica thin film for the selective recovery of low molecular weight proteins and peptides from human serum. The physico-chemical properties of our mesoporous chips were finely tuned to provide substantial control in peptide enrichment and consequently profile the serum proteome for diagnostic purposes. Medicine Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice Wesley S. Bond1,2, Lalita Wadhwa2,3, Laszlo Perlaky2,3, Rebecca L. Penland4, Mary Y. Hurwitz2,3, Richard L. Hurwitz*2,3,5,6, Patricia Chèvez-Barrios*4,5,7 1Interdepartmental Program in Translational Biology & Molecular Medicine, Baylor College of Medicine, 2Texas Children's Cancer Center, Baylor College of Medicine, 3Department of Pediatrics, Baylor College of Medicine, 4Department of Pathology, The Methodist Hospital Research Institute, 5Department of Ophthalmology, Retinoblastoma Center of Houston, 6Baylor College of Medicine, Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy, Baylor College of Medicine A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres. Biology Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure Kenneth K. Kwong1,2, Lenuta Kloetzer1,2,3,4, Kelvin K. Wong5,6, Jia-Qian Ren1,2, Braden Kuo1,2,3,4, Yan Jiang7, Y. Iris Chen1,2, Suk-Tak Chan1,2,8, Geoffrey S. Young9, Stephen T.C. Wong5,6 1Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 2Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, 3Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, 4Department of Medicine, Massachusetts General Hospital, Harvard Medical School, 5Center for biotechnology and Informatics, The Methodist Hospital Research Institute, 6Department of Radiology, The Methodist Hospital, Weill Cornell Medical College, 7Bejing University of Chinese Medicine, 8Department of Health Technology and Informatics, The Hong Kong Polytechnic University, 9Department of Radiology, Brigham and Women's Hospital, Harvard Medical School Gua Sha, traditional Chinese therapeutic skin scraping, causes subcutaneous microvascular blood extravasation. We report a protocol of bioluminescence imaging of HO-1-luciferase transgenic mice to demonstrate that Gua Sha upregulates heme oxygenase-1 (HO-1) in multiple organs.