Seattle Children's Hospital 2 articles published in JoVE Biology An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation Mingxiao Feng1, Sara Divall3, Sheng Wu1,2 1Department of Pediatrics, Johns Hopkins University School of Medicine, 2Department of Physiology, Temple University School of Medicine, 3 Primary hepatocytes are a valuable tool to study liver response and metabolism in vitro. Utilizing commercially available reagents, an improved time- and labor-efficient protocol for mouse primary hepatocyte isolation was developed. Developmental Biology Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells Ashley Ulm1, Christopher N. Mayhew2, Jason Debley3, Gurjit K. Khurana Hershey4, Hong Ji1,4 1Division of Developmental Biology, Cincinnati Children's Hospital, 3Division of Asthma Research, Cincinnati Children's Hospital This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).