Universidad Icesi 1 article published in JoVE Biology Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification Steven A. David-Jimenez1, Paola A. Caicedo1, Maria F. Villegas-Torres1,2, Natalia Campillo-Pedroza1,3 1Facultad de Ingeniería, Diseño y Ciencias Aplicadas, Universidad Icesi, 2Centro de Investigaciones Microbiológicas (CIMIC), Department of Biological Sciences, Universidad de los Andes, 3BioDx: Diagnóstico y Soluciones Biotecnológicas S.A.S, Universidad Icesi Here we provide a complete protocol to standardize and implement the method for detecting the SARS-CoV-2 virus in human samples by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method, done within 60 min, could be adapted to any laboratory or point-of-care at a low cost and using inexpensive equipment.
