Universidad de Ibague 1 article published in JoVE Environment Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images José A. Fernandez-Gallego1,2,3, María Luisa Buchaillot1,2, Adrian Gracia-Romero1,2, Thomas Vatter1,2, Omar Vergara Diaz1,2, Nieves Aparicio Gutiérrez4, María Teresa Nieto-Taladriz5, Samir Kerfal6, Maria Dolors Serret1,2, José Luis Araus1,2, Shawn C. Kefauver1,2 1Plant Physiology Section, Faculty of Biology, University of Barcelona, 2Agrotecnio, 3Programa de Ingeniería Electrónica, Facultad de Ingeniería, Universidad de Ibagué, 4Instituto Tecnológico Agrario de Castilla y León (ITACyL), 5Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 6Syngenta Spain We present a protocol for counting durum wheat and barley ears, using natural color (RGB) digital photographs taken in natural sunlight under field conditions. With minimal adjustments for camera parameters and some environmental condition limitations, the technique provides precise and consistent results across a range of growth stages.