All Protocols and Video Articles for scientific research | JoVE

Showing 1 - 12 of 158 videos for "Cell Membrane Permeabilization"

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Journal (Bioengineering)

Production of Recombinant AAV by Transient Transfection

Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^2,4, Carina Henriques^2,5, Ana C. Silva^2,3, Diana D. Lobo^2,3, Luísa Cortes^2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC - Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB - Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC - Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector - Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC - Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC - Microscopy Imaging Center of Coimbra - CNC,University of Coimbra

Journal (Biology)

Visualization of the DNA Hybrids Using Chemiluminescence for Leptospira Detection in Water Samples

Eduardo Delgadillo-Tellez, Beatriz Zavaleta-Villa, Daniel Atilano-López, Rigoberto Hernández-Castro, Angélica Olivo-Díaz, Erika Margarita Carrillo-Casas

¹Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, ³Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Journal (Biology)

DNA-Membrane Fixation and Probe Hybridisation for Leptospira Detection in Water Samples

Eduardo Delgadillo-Tellez, Beatriz Zavaleta-Villa, Daniel Atilano-López, Rigoberto Hernández-Castro, Angélica Olivo-Díaz, Erika Margarita Carrillo-Casas

¹Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, ³Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Journal (Biology)

Amplification of Leptospira DNA and its Transfer on the Membrane Using a Dot-Blot Apparatus

Eduardo Delgadillo-Tellez, Beatriz Zavaleta-Villa, Daniel Atilano-López, Rigoberto Hernández-Castro, Angélica Olivo-Díaz, Erika Margarita Carrillo-Casas

¹Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, ³Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Journal (Biology)

Lactobacillaceae Sample Preparation for Flow Cytometric Analysis

Ronit Suissa, Uzi Hadad, Michael Meijler, Ilana Kolodkin-Gal⁴

¹Department of Chemistry,Ben-Gurion University of the Negev, ²Ilse Kats Institute for Nanoscale Science & Technology,Ben-Gurion University of the Negev, ³Department of Plant Pathology and Microbiology, Faculty of Agriculture, Food and Environment,The Hebrew University of Jerusalem, ⁴The Scojen institute for synthetic biology,Reichman university

Journal (Bioengineering)

2D Expansion, 2D Stimulation, and 3D Organoid Maturation of Adult Normal Human Dermal Fibroblasts for Tendon Research and Engineering

Ana Luísa Graça, Niklas Kroner-Weigl, Viviana Reyes Alcaraz, Sigrid Müller-Deubert, Maximilian Rudert, Denitsa Docheva

¹Department of Musculoskeletal Tissue Regeneration, Orthopaedic Hospital König-Ludwig-Haus,University of Würzburg, ²Laboratory for Experimental Trauma Surgery, Department of Trauma Surgery,University Hospital Regensburg, ³Department of Orthopaedics, Orthopaedic Hospital König-Ludwig-Haus,University of Würzburg

Journal (Bioengineering)

Bacterial Inoculation of Organ Chips with Differentiated Human Vaginal Epithelial Cells for Studying Living Vaginal Microbiome Under Microaerophilic Conditions

Aakanksha Gulati, Alicia Jorgenson, Abidemi Junaid, Donald E. Ingber^2,3

¹Wyss Institute for Biologically Inspired Engineering,Harvard University, ²Vascular Biology Program and Department of Surgery,Boston Children's Hospital and Harvard Medical School, ³Harvard John A. Paulson School of Engineering and Applied Sciences

Journal (Bioengineering)

Connecting Human Uterine Fibroblast-Seeded Organ Chips to Pods and Differentiation of Vaginal Epithelial Cells for Studying Living Vaginal Microbiome

Aakanksha Gulati, Alicia Jorgenson, Abidemi Junaid, Donald E. Ingber^2,3

¹Wyss Institute for Biologically Inspired Engineering,Harvard University, ²Vascular Biology Program and Department of Surgery,Boston Children's Hospital and Harvard Medical School, ³Harvard John A. Paulson School of Engineering and Applied Sciences

Journal (Bioengineering)

Seeding Organ Chips with Human Uterine Fibroblasts (HUFs) for Studying Living Vaginal Microbiome

Aakanksha Gulati, Alicia Jorgenson, Abidemi Junaid, Donald E. Ingber^2,3

¹Wyss Institute for Biologically Inspired Engineering,Harvard University, ²Vascular Biology Program and Department of Surgery,Boston Children's Hospital and Harvard Medical School, ³Harvard John A. Paulson School of Engineering and Applied Sciences

Journal (Biology)

Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies

Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

Journal (Biology)

Preclearing of Transfected HEK293 Protein with the Protein G Gel and Capturing Protein Complexes Using Epitope Tag Affinity Gel

Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

Journal (Biology)

Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation

Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

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