免疫学和感染,第147期,人类,免疫抑制,T细胞,移植物对宿主疾病,异种性GVHD,数字PCR,CD3 Protocols and Video Articles

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Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR

Authors

Author

Amara Seng¹, Mary A. Markiewicz¹

Institution

¹Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center

Journal (Immunology and Infection)

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Authors

Author

Alessandra Sottini¹, Federico Serana¹, Diego Bertoli¹, Marco Chiarini¹, Monica Valotti¹, Marion Vaglio Tessitore¹, Luisa Imberti¹

Institution

¹CREA, Diagnostics Department, Spedali Civili di Brescia

Journal (Immunology and Infection)

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Authors

Author

Bryan A. Anthony¹, Gregg A. Hadley¹

Institution

¹Department of Surgery, The Ohio State University Medical Center

Journal (Immunology and Infection)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Authors

Author

Hans U. Graber¹

Institution

¹Institute for Food Sciences IFS, Agroscope

Journal (Immunology and Infection)

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Authors

Author

Shi Zhong¹, Karolina Malecek^1,2, Arianne Perez-Garcia¹, Michelle Krogsgaard¹

Institution

¹NYU Cancer institute, New York University School of Medicine, ²Program in Structural Biology, New York University School of Medicine

Journal (Immunology and Infection)

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Authors

Author

Angelika Schmidt¹, Szabolcs Éliás*¹, Rubin N. Joshi*¹, Jesper Tegnér¹

Institution

¹Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

Journal (Immunology and Infection)

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Education

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PCR - Polymerase Chain Reaction

JoVE Core (Core: Biologia molecular)

PCR

JoVE Core (Core: Biologia)

Real Time RT-PCR

JoVE Core (Core: Biologia molecular)

PCR: The Polymerase Chain Reaction

Science Education (Basic Biology)

Student t Distribution

JoVE Core (Core: Estatística)

RNA Analysis of Environmental Samples Using RT-PCR

Science Education (Environmental Sciences)

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