发育生物学，第123期，细胞培养，单层扩增，软骨细胞去分化，DNA甲基化，5-mC斑点印迹分析，基因组DNA提取 Protocols and Video Articles

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A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Authors

Author

Zhaofeng Jia^1,2, Yujie Liang³, Bin Ma^4,5, Xiao Xu^2,6, Jianyi Xiong², Li Duan², Daping Wang^1,2

Institution

¹Guangzhou Medical University, ²Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), ³Department of Chemistry, The Chinese University of Hong Kong, ⁴School of Biomedical Engineering, Shanghai Jiao Tong University, ⁵Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, ⁶Shantou University Medical College

Journal (Developmental Biology)

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Authors

Author

Yujing Li¹, Chun-Xiao Song², Chuan He², Peng Jin¹

Institution

¹Department of Human Genetics, Emory University School of Medicine, ²Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago

Journal (Biology)

5-Methylcytosine Dot Blot to Determine the Extent of DNA Methylation

5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells (Video) | JoVE

Authors

Author

Lukasz S. Borowski^1,2, Karolina Kasztelan^2,3, Jolanta Czerwinska-Kostrzewska^1,2, Roman J. Szczesny²

Institution

¹Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, ²Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

Journal (Biochemistry)

DNA Stable-Isotope Probing (DNA-SIP)

Authors

Author

Eric A. Dunford¹, Josh D. Neufeld¹

Institution

¹Department of Biology, University of Waterloo

Journal (Biology)

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Education

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DNA Microarrays

JoVE Core (Core: Biologia molecular)

Recombinant DNA

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DNA Helicases

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Complementary DNA

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DNA Packaging

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DNA Replication

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