ホルムアルデヒド架橋線虫の超音波処理とクロマチン免疫沈降

Published: May 05, 2023

doi:

10.3791/200310

Chengyin Li*¹, Phoebe A. W. Bhagoutie*¹, Victor Lao, Arneet L. Saltzman

¹Department of Cell and Systems Biology,University of Toronto

Tags

Keywords: Sonication Formaldehyde-Crosslinked Caenorhabditis Elegans Chromatin Immunoprecipitation Resuspension Buffer Detergents Lysate Centrifugation Immunoprecipitation Anti-H3K9 Trimethylation Anti-histone H3 IgG Protein G Magnetic Beads FA-150 Buffer Chromatin IP Elution Buffer Reverse Cross-linking DNA Elution

Play Video

PDF

DOI

Citar este artigo

Sonication of Formaldehyde-Crosslinked Caenorhabditis elegans and Chromatin Immunoprecipitation. J. Vis. Exp. (Pending Publication), e200310, doi:10.3791/200310 (2023).

ホルムアルデヒド架橋線 虫 の超音波処理とクロマチン免疫沈降

Tags

Play Video

Citar este artigo

View Video

✖

To prove you're not a robot, please enter the text in the image below

ホルムアルデヒド架橋線虫の超音波処理とクロマチン免疫沈降