Detecção de proteínas em células de adenocarcinoma gástrico transfectado TMEM200A siRNA usando Western Blotting

Published: September 15, 2023

doi:

10.3791/200744

Yaowen Zhang, Shanshan Kuang, Hancheng Qin, Na Zhao, Yixin Yang, Jisheng Xie

¹Department of Histology and Embryology,Youjiang Medical University for Nationalities, ²Department of Pathophysiology,Youjiang Medical University for Nationalities

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Keywords: TMEM200A SiRNA Stomach Adenocarcinoma Western Blotting Protein Detection Cell Culture PBS Radioimmune Precipitation Assay BCA Protein Assay SDS-PAGE PVDF Membrane TBST Blocking Buffer Primary Antibody Secondary Antibody ECL Substrate Epithelial Mesenchymal Transition Phosphoinositide 3-kinase Signaling Pathway

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Protein Detection in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells Using Western Blotting. J. Vis. Exp. (Pending Publication), e200744, doi:10.3791/200744 (2023).

Detecção de proteínas em células de adenocarcinoma gástrico transfectado TMEM200A siRNA usando Western Blotting

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