ウェスタンブロッティングを用いた TMEM200A siRNAトランスフェクション胃腺癌細胞におけるタンパク質検出(英語)

Published: September 15, 2023

doi:

10.3791/200744

Yaowen Zhang, Shanshan Kuang, Hancheng Qin, Na Zhao, Yixin Yang, Jisheng Xie

¹Department of Histology and Embryology,Youjiang Medical University for Nationalities, ²Department of Pathophysiology,Youjiang Medical University for Nationalities

Tags

Keywords: TMEM200A SiRNA Stomach Adenocarcinoma Western Blotting Protein Detection Cell Culture PBS Radioimmune Precipitation Assay BCA Protein Assay SDS-PAGE PVDF Membrane TBST Blocking Buffer Primary Antibody Secondary Antibody ECL Substrate Epithelial Mesenchymal Transition Phosphoinositide 3-kinase Signaling Pathway

Play Video

PDF

DOI

Citar este artigo

Protein Detection in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells Using Western Blotting. J. Vis. Exp. (Pending Publication), e200744, doi:10.3791/200744 (2023).

ウェスタンブロッティングを用いた TMEM200A siRNAトランスフェクション胃腺癌細胞におけるタンパク質検出(英語)

Tags

Play Video

Citar este artigo

View Video

✖

To prove you're not a robot, please enter the text in the image below