Дифференцировка плюрипотентных стволовых клеток человека in vitro в инсулин-продуцирующие клетки поджелудочной железы
1Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Cell Biology and Functional Genomics, 2Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center,Columbia University, 3Department of Diabetes,Imperial College Healthcare NHS Trust, 4Faculté de Médicine,Université de Montréal, 5Lee Kong Chian Imperial Medical School,Nanyang Technological University
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In VitroHuman Pluripotent Stem CellsInsulin-producingPancreatic CellsCell PassageCoating SolutionDissociation SolutionTrypan BlueHemocytometerRock InhibitorBasal MediumSupplementsWashing MediumDay One MediumPancreatic Progenitor MediumDissociation BufferMicrowellsAnti-Adherence Rinsing Solution
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In Vitro Human Pluripotent Stem Cell Differentiation into Insulin-Producing Pancreatic Cells. J. Vis. Exp. (Pending Publication), e200998, doi:10.3791/200998 (2024).