JoVE Journal > Biology

In Vitro Human Pluripotent Stem Cell Differentiation into Insulin-Producing Pancreatic Cells

Published: February 02, 2024

doi:

Ines Cherkaoui, Qian Du, Dieter Egli, Shivani Misra³, Guy A. Rutter^4,5

¹Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Cell Biology and Functional Genomics, ²Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center,Columbia University, ³Department of Diabetes,Imperial College Healthcare NHS Trust, ⁴Faculté de Médicine,Université de Montréal, ⁵Lee Kong Chian Imperial Medical School,Nanyang Technological University

Tags

Keywords: In Vitro Human Pluripotent Stem Cells Insulin-producing Pancreatic Cells Cell Passage Coating Solution Dissociation Solution Trypan Blue Hemocytometer Rock Inhibitor Basal Medium Supplements Washing Medium Day One Medium Pancreatic Progenitor Medium Dissociation Buffer Microwells Anti-Adherence Rinsing Solution

Play Video

DOI

Citar este artigo

In Vitro Human Pluripotent Stem Cell Differentiation into Insulin-Producing Pancreatic Cells. J. Vis. Exp. (Pending Publication), e200998, doi:10.3791/200998 (2024).

View Video