Intraperitoneal Injection: A Method of Solution Delivery into the Abdominal Cavity of an Adult Zebrafish
Published: April 30, 2023
Abstract
Source: Luukinen, H., et al. Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish. J. Vis. Exp. (2018).
This video describes a technique of intraperitoneal injection, a method for delivering solutions into the abdominal cavity of Adult Zebrafish.
Protocol
1. Experimental M. marinum Infection with Intraperitoneal Injection Pipette a 5 µL droplet of the diluted bacterial solution onto a piece of parafilm film and pull the droplet into a 30 G insulin needle. Use 5–8 month-old wild-type fish and rag1−/−hu1999 mutant fish for the experiment. Anesthetize adult zebrafish in the tank water with 0.02% 3-aminobenzoic acid ethyl ester (pH 7.0). Position the fish ventral side up into a slit on a moist foamed plastic. …
Materials
| Mycobacterium marinum | American Type Culture Collection | ATCC 927 | |
| Phenol red | Sigma-Aldrich | P3532 | |
| Phosphate buffered saline tablets (PBS) | Sigma-Aldrich | P4417-50TAB | |
| Middlebrock 7H10 agar | BD, Thermo Fisher Scientific | 11799042 | |
| 3-aminobenzoic acid ethyl ester (pH 7.0) | Sigma-Aldrich | A5040 | |
| Omnican 100 30 G insulin needle | Braun | 9151133 | |
| 1 mL syringe | Henke Sass Wolf | 4010.200V0 |
Citar este artigo
Intraperitoneal Injection: A Method of Solution Delivery into the Abdominal Cavity of an Adult Zebrafish. J. Vis. Exp. (Pending Publication), e20195, doi: (2023).