This video describes the surface-enhanced resonance Raman scattering technique to detect metastatic ovarian cancer using targeted nanoprobes specific anti-receptor antibodies and non-targeted control nanoprobes. This method helps to determine the relative abundance of the two nanoprobes and differentiate the cancer cells from the visceral background.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Gold Nanostar Core Synthesis NOTE: Gold nanostars are used as cores for both flavors of SERRS nanoprobes used in this experiment. Prepare 800 mL of 60 mM ascorbic acid (C6H8O6) solution in deionized (DI) water and 8 mL of 20 mM tetrachloroauri…
Antibody-Functionalized SERRS Nanoprobe Based Imaging: A Highly Sensitive Raman-imaging Technique to Detect Metastatic Ovarian Cancer In Vivo. J. Vis. Exp. (Pending Publication), e20373, doi: (2023).