This video describes the procedure for orthotopic inoculation of thyroid cancer cells into the thyroid gland of mouse models, which may then serve as a valuable platform to assess carcinogenesis and discover potential treatment strategies.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Thyroid Orthotopic Inoculation of Cells Cell preparation Engineer a Cal62 human thyroid cancer cell line to overexpress a transgenic construct that constitutively expresses GFP and luciferase. Select the transgenic cells by antibiotic resistance or by sorting the GFP-positive cells with flow cytometry….
Declarações
The authors have nothing to disclose.
Materials
Basement Membrane Matrix: Matrigel Basement Membrane Matrix High Concentration
Corning
#354248
In vivo delivery reagent: Invivofectamine 3.0 Reagent
Thermo Fisher
IVF3005
In vivo imaging software: IVIS-Lumina II Imaging System
Orthotopic Inoculation of Thyroid Cancer Cells into Murine Thyroid: A Procedure to Establish Orthotopic Thyroid Cancer Mouse Model. J. Vis. Exp. (Pending Publication), e20480, doi: (2023).