Spinal Cord Extraction: A Protocol to Isolate Murine Spinal Cord for In Vitro Studies
Published: April 30, 2023
Abstract
Source: Na'ara, S. et al. In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model. J. Vis. Exp. (2016)
In this video, we describe a step-by-step protocol to isolate spinal cord from a mouse model for in vitro studies.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Harvesting the Spinal Cord Euthanize the mouse using a CO2 chamber. Avoid cervical dislocation as it might cause damage to the ganglion roots due to shear forces. From here on, perform all steps under sterile conditions. Soak the animal with 70% ethanol. This step is important for sterilization…
Declarações
The authors have nothing to disclose.
Materials
| Forceps | Sigma-Aldrich | F4142 | |
| Surgical blade | Sigma-Aldrich | Z309036 | |
| Scissors | Sigma-Aldrich | S3271 | |
| 35 mm Petri dishes, glass bottom | de groot | 60-627860 | |
| 70% ethanol | Sigma | ||
| Cold PBS | Biological industries | 02-023-1A |
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Spinal Cord Extraction: A Protocol to Isolate Murine Spinal Cord for In Vitro Studies. J. Vis. Exp. (Pending Publication), e20489, doi: (2023).