Colonoscopic Guided Pinch Biopsy in a Mouse Model: A Technique to Visualize and Extract Tissue Samples from Mouse Colon

Abstract

Source: Montrose, D. C., et al. Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes. J. Vis. Exp. (2021)

This video demonstrates the protocol for performing colonoscope-guided pinch biopsy in the mouse. This technique helps visually analyze the colon lumen and collect tissue samples for detailed studies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Colonoscopy and wound induction Pre-assemble the components of the endoscope by first inserting the 1.9 mm rigid bore endoscope into the sheath (Figure 1A-B). Attach the air pump (to provide colonic insufflation) using the tubing provided, to the gas valve on the left side of the sheath next to the working channel (<str…

Representative Results

Figure 1: Items needed to carry out biopsies. (A) Image of lens (a), sheath (b) and biopsy forceps (c). (B) Insertion of the lens into the sheath. (C) Insertion of forceps through the working channel of the sheath (solid arrow). Dashed arrow indicates correct location for attachment of air pump.

Materials

Biopsy forceps, 3 Fr	Karl Storz	61071ZJ
Coloview Tower system	Karl Storz	contact company
Examination sheath, 9 Fr, Kit	Karl Storz	61029DK
Hopkins telescope, 0', 1.9 mm x 10 cm	Karl Storz	64301AA
Isofluorane	Covetrus	2905
Pawfly MA-60 aquarium pump	Amazon	N/A