Papanicolaou Staining: A Technique to Visualize the Cytological Features of Tubal Cells
Published: April 30, 2023
Abstract
Source: Chen, H. et al. Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection. J. Vis. Exp. (2017).
In this video, we demonstrate a modified protocol of Papanicolaou staining to study tubal cytology. This technique can be used to visualize the cytological features of malignant and non-malignant fallopian tube epithelial cells.
Protocol
1. Modified Papanicolaou Staining Protocol Stain the tissue smeared slides by running the automatized non-gyn stain procedure, as illustrated in Table 1. NOTE: The staining method represents a modification of the Papanicolaou staining technique that we use routinely for non-gynecologic specimens. We use an automated tissue processor to stain some slides. We chose the Non-Gyn staining procedure over the Gyn staining procedure in this study. The Eosin used is EA-65,…
Declarações
The authors have nothing to disclose.
Materials
| Papanicolaou Stain, EA 65 | sigma aldrich | HT40432 | |
| 95% Ethanol | sigma aldrich | 652261 | |
| 100% Ethanol | sigma aldrich | 493538 | |
| Xylene | sigma aldrich | 214736 | |
| Hematoxylin | sigma aldrich | H9627 |
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Papanicolaou Staining: A Technique to Visualize the Cytological Features of Tubal Cells. J. Vis. Exp. (Pending Publication), e21052, doi: (2023).