The video describes a single-cell western blotting technique that identifies the composition of individual cells captured on a single-cell western blotting chip in a heterogeneous suspension. The technique detects the fluorescence intensity of the fluorophore tagged to a protein marker specific to the target cell.
Protocol
1. Determining Cell Composition in the Cell Suspension Using Single-cell Western Blot (scWB) Analysis Rehydrate the scWB chip before use in 15 mL of 1x suspension buffer in a Petri dish for at least 10 min at RT. Add 1 mL of the diluted single-cell suspension (10,000-100,000 cells/mL) to the rehydrated chip. Set the surface onto the bottom of a 10 cm Petri dish. Ensure that the surface is set flat. Cover the Petri dish with a lid to prevent drying and let the cells settle for 5-15 min by a…