Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy

Abstract

Source: Anderson, L. S. et al., A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection. J. Vis. Exp. (2019)

This video demonstrates a punch biopsy procedure to create a precise wound in a mouse model. The excision exposes the subcutaneous layer of the skin, providing a suitable site for studying immune responses to localized pathogen infection.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Mouse Source and Housing Derive Lysozyme M- enhanced green fluorescent protein (LysM-eGFP) mice on a C57BL/6J genetic background as described previously. Derive LysM-EGFP× myeloid differentiation primary response 88 (MyD88)-/- mice by crossing LysM-EGFP mice with MyD88-/- mice on a C57BL/6J background. Ho…

Materials

6mm Disposable Biopsy Punch	Integra Miltex	33-36
Buprenoprhine hydrochloride injectable	Western Medical Supply	7292	0.3 mg/mL
C57BL/6J Mice	Jackson Labratory	664
LysM-eGFP Mice	Thomas Graff Albert Einstein College of New York	NA
MyD88 KO Mice	Jackson Labratory	9088
Non-woven sponges	AMD- Ritmed Inc	A2101-CH	5 cm x 5 cm
Povidone Iodine 10% Solution	Aplicare	697731