The video demonstrates locoregional delivery of oncolytic viruses through hepatic arterial injection as a therapeutic approach for hepatocellular carcinoma. These viruses selectively replicate in tumor cells, inducing cell lysis and releasing tumor cell-associated antigens. This process triggers immunogenic cell death, inducing an anti-tumor response.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparations before beginning surgery Sterilize surgical instruments (i.e., by autoclaving). Dilute the virus to the appropriate concentration, which will vary depending on the toxicity of the specific virus being used and the susceptibility of the rat strain. NOTE: For example…
Representative Results
Figure 1. Photographs of the hepatic arterial infusion procedure. A laparotomized rat demonstrates the placement of the abdominal wall retractor and gauze swabs (A). After lifting out the hepatic lobes, the common hepatic artery (designated CHA) and gastroduodenal artery (designated GA) are identified and dissected (B). After ligating the distal end of the gastro…
Declarações
The authors have nothing to disclose.
Materials
Veterinary clippers
Aesculap
GT415
Small, cordless trimmer ideal for removing fur from surgical area
Stereomicroscope
Zeiss
Stemi SV6
30G Needles
Braun
4656300
30G x ½"
1ml syringes
Braun
9161406V
Tuberculin syringe
Disposable scalpel
Feather
2975#15
#15 blade
Standard surgical scissors
Fine Science Tools
14001-13
Sharp/blunt, for opening skin and muscle
Adson forcep
Fine Science Tools
1101-12
With teeth, for grasping skin and muscle
Alm retractor
Fine Science Tools
17008-07
With blunt teeth, for spreading abdominal cavity open during surgery
Gauze swabs
Lohmann & Rauscher
18504
7.5 x 7.5 cm, should be autoclaved prior to use
Cotton-tipped applicator swabs
Lohmann & Rauscher
11970
Sterile
Fine-tipped foreceps
Fine Science Tools
11063-07
0.4mm, angled tip, for dissecting hepatic artery
Vannas spring scissors
Fine Science Tools
91500-09
For delicate cutting
Micro-needle holder
Fine Science Tools
12076-12
For ligating gastroduodenal artery
Needle holder
Fine Science Tools
12005-15
Tungsten carbide jaws
7-0 Prolene sutures
Ethicon
8648H
Polypropylene suture with curved needle, for ligating gastroduodenal artery