This video illustrates a technique for isolating cranial neural folds from the midbrain region of chick embryos. It presents a detailed dissection process of the neural folds, their placement, and attachment on fibronectin-coated coverslips, followed by the observation of neural crest cell migration from the neural folds.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of solutions and materials Prepare Ringer's solution by mixing 123.3 mM NaCl, 1.53 mM CaCl2, 4.96 mM KCl, 0.809 mM Na2HPO4, and 0.147 mM KH2PO4 (see Table of Materials). Adjust pH to 7.4 and filter sterilize into 100 mL bottles. Stor…
Representative Results
Figure 1. Dissection of chick dorsal neural folds. Working in Ringer's P/S, spring scissors were used to excise neural folds. (A) Embryo dorsal view, anterior toward the top of the figure. Neural folds appear more opaque than the surrounding tissue. Midbrain neural folds lie posterior to the optic lobes (pink) and anterior to the cardiac crescent (yellow). (B)</strong…
Declarações
The authors have nothing to disclose.
Materials
AxioObserver equipped with an LSM710 confocal scan head controlled by ZEN 3.0 SR software
Zeiss
Used alpha Plan-Apochromat 100x/1.46 Oil DIC M27 objective