En Laser-induceret musemodel af kronisk okulær hypertension til Karakterisere synsfejl
Summary
Kronisk okulær hypertension fremkaldes ved hjælp laserfotokoagulation af trabekelværket i muse øjne. Det intraokulære tryk (IOP) er forhøjet i flere måneder efter laserbehandling. Faldet af visuel skarphed og kontrast følsomhed forsøgsdyr overvåges ved hjælp af optomotor testen.
Abstract
Glaukom, ofte forbundet med forhøjet intraokulært tryk (IOP), er en af de førende årsager til blindhed. Vi søgte at etablere en musemodel af okulær hypertension at efterligne human højspænding glaukom. Her laserbelysning påføres hornhindens limbus at photocoagulate den vandige udstrømning, inducere nævervinklet. Ændringerne i IOP overvåges ved hjælp af en rebound tonometer før og efter laserbehandlingen. En optomotor adfærdsmæssige test anvendes til at måle tilsvarende ændringer i visuel kapacitet. Den repræsentativt resultat fra en mus, der udviklede vedvarende IOP elevation efter laserbelysning vises. En nedsat synsstyrke og kontrast følsomhed er observeret i denne okular hypertensive mus. Sammen vores undersøgelse indfører en værdifuld modelsystem til at undersøge neuronal degeneration og de underliggende molekylære mekanismer i glaucomatøse mus.
Protocol
Representative Results
Discussion
Vi rapporterer over at vedvarende okulær hypertension kan induceres ved laserbelysning i muse øjne. Sammenlignet med saltvand injektion model 18 og venen cautery model 11 begge kræver omfattende mikrokirurgiske færdigheder er laserbelysning forholdsvis enkel og let at udføre. Normalt kan vi udføre laserbelysning til 4-6 mus i 2-3 timer. De kritiske skridt til at opnå vedvarende IOP elevation er forkammeret udfladning før laser og parametrene for laserbelysning. Tømning væsken ud i forkamm…
Declarações
The authors have nothing to disclose.
Acknowledgements
Det arbejde, der er indeholdt i dette papir er blevet støttet af Dr. Douglas H. Johnson Award for Glaukom Research fra det amerikanske Health Assistance Foundation (XL), William & Mary Greve Special Scholar Award fra forskning for at forebygge blindhed (XL), den Illinois Society for forebyggelse af blindhed (HC) og NIH tilskud R01EY019034 (XL).
Materials
| Reagent | |||
| moxifloxacin | Alcon Labs, Inc. | NDC 0065-4013-03 | 0.5 %, Rx only |
| Proparacaine Hydrochloride | Bausch & Lomb | NDC 24208-730-06 | 0.5 %, Rx only |
| Ophthalmic Solution USP | Bausch & Lomb | NDC 24208-730-06 | .5 %, Rx only |
| ketamine | Butler Schein Animal Health | NDC 11695-0550-1 | 100 mg / kg |
| xylazine | LLOYD Inc. of Iowa | NADA 139-236 | 10 mg / kg |
| atropine sulfate solution | Alcon Labs, Inc. | NDC 61314-303-02 | 1 %, Rx only |
| Equipment | |||
| Slit Lamp, TOPCON | Visual Systems Inc | SL-3E | powered by PS-30A |
| OptoMotry 1.8.0 virtual | CerebralMechanics Inc. | ||
| opto-kinetic testing system | CerebralMechanics Inc. | ||
| Tonometer, TonoLab, for mice | Colonial Medical Supply | ||
| Heating pad | Sunbeam Products Inc | 722-810 | |
| Argon laser | Coherent Inc | Ultima 2000SE | |
| DECAPICONE Plastic cone holder | Braintree Sci Inc. | MDC-200 | for mouse |
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