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Single-cell RNA-Seq af definerede subsæt af retinale Ganglion-celler
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JoVE Journal Neuroscience
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq af definerede subsæt af retinale Ganglion-celler

DOI:
10.3791/55229-v

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11:26 min

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May 22, 2017

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, , , ,
1Department of Genetics, Development, and Cell Biology,Iowa State University, 2Department of Neurobiology,Northwestern University

Chapters

  • 00:05Title
  • 01:14Preparation of Retinal Tissue
  • 03:19Visualization and Targeting of GFP+ Retinal Ganglion Cells
  • 04:30Cell Isolation
  • 05:50RNA Purification
  • 08:38Results: Evaluation of Cell Isolation and Sequencing Preparation
  • 10:28Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en kombinatorisk tilgang til klassificering af neuronelle celletyper forud for isolation og til den efterfølgende karakterisering af enkeltcelletranscriptomer. Denne protokol optimerer forberedelsen af ​​prøver til vellykket RNA-sekventering (RNA-Seq) og beskriver en metodologi, der er designet specielt til forbedret forståelse af cellemangfoldighed.

Tags

Single-cell RNA-SeqRetinal Ganglion CellsFluorescent LabelingNeuronal SubtypeCellular DiversityRNA IsolationPatch-clampElectrophysiological RecordingsMicropipette PullerExtracellular SolutionOxygenationFluorescent Microscopy

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