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视网膜神经节细胞定义子集的单细胞RNA-Seq
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Neuroscience
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JoVE Journal Neuroscience
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
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视网膜神经节细胞定义子集的单细胞RNA-Seq

DOI:
10.3791/55229-v

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11:26 min

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May 22, 2017

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, , , ,
1Department of Genetics, Development, and Cell Biology,Iowa State University, 2Department of Neurobiology,Northwestern University

Chapters

  • 00:05Title
  • 01:14Preparation of Retinal Tissue
  • 03:19Visualization and Targeting of GFP+ Retinal Ganglion Cells
  • 04:30Cell Isolation
  • 05:50RNA Purification
  • 08:38Results: Evaluation of Cell Isolation and Sequencing Preparation
  • 10:28Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一种组合方法，用于在分离之前对神经元细胞类型进行分类，以及随后对单细胞转录组进行表征。该协议优化了用于成功的RNA测序（RNA-Seq）的样品的制备，并描述了专门为增强细胞多样性的理解而设计的方法。

Tags

Single-cell RNA-SeqRetinal Ganglion CellsFluorescent LabelingNeuronal SubtypeCellular DiversityRNA IsolationPatch-clampElectrophysiological RecordingsMicropipette PullerExtracellular SolutionOxygenationFluorescent Microscopy

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