1. Optional in the case that the tissue is fibrous: Centrifuge the solution at 750 x g for 4 min.
Protocol
A correction was made to: Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration. An optional step was added to step 7 of the 'Mitochondrial Isolation' section of the protocol. The step is: 1. Optional in the case that the tissue is fibrous: Centrifuge the solution at 750 x g for 4 min.
Erratum: Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration. J. Vis. Exp. (107), e5763, doi: (2016).