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Isolering myofibrils fra skeletmuskulatur biopsier og bestemmelse kontraktile funktion med en Nano-Newton resolution Force Transducer
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Isolating Myofibrils from Skeletal Muscle Biopsies and Determining Contractile Function with a Nano-Newton Resolution Force Transducer

Isolering myofibrils fra skeletmuskulatur biopsier og bestemmelse kontraktile funktion med en Nano-Newton resolution Force Transducer

DOI:
10.3791/61002-v

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07:55 min

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May 07, 2020

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, , , ,
1Department of Physiology,Amsterdam UMC, 2Department of Kinesiology and Physical Education, Faculty of Education,McGill University, 3IONOptix BV

Chapters

  • 00:04Introduction
  • 00:50Myofibril and Mounting Needle Preparation
  • 02:08Experiment Initialization
  • 04:03Tension Redevelopment Rate Measurement
  • 05:04Stepwise Stretch
  • 05:33Results: Representative Active and Passive Tension Analyses
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

Præsenteret her er en protokol til at vurdere kontraktile egenskaber af striated muskel myofibrils med nano-Newton opløsning. Protokollen anvender en opsætning med en interferometribaseret, optisk kraftsonde. Denne opsætning genererer data med et højt signal-støj-forhold og gør det muligt at vurdere myofibrils kontraktile kinetik.

Tags

MyofibrilsSkeletal Muscle BiopsiesContractile FunctionNano-Newton Resolution Force TransducerSarcomere DysfunctionSarcomeric ProteinsPermeabilized CardiomyocytesHomogenizationMyofibril SuspensionRelaxing SolutionPoly-HEMA Coated SlideShellac Ethanol GlueMounting NeedleMicro-manipulatorsSarcomere LengthVessel FunctionVideo ImagingMicroscope Stage

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