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Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts
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Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

DOI:
10.3791/61127-v

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06:27 min

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May 16, 2020

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, , ,
1Department of Genetics,St. Jude Children’s Research Hospital

Chapters

  • 00:05Introduction
  • 00:45Primary Fibroblast Isolation and Culture
  • 03:04Purification of Exosomes Using Differential and Ultra-Centrifugation
  • 04:58Results: Characterization of Purified Exosomes
  • 05:47Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol illustrates the 1) the isolation and culture of primary fibroblasts from the adult mouse gastrocnemius muscle as well as 2) purification and characterization of exosomes using a differential ultracentrifugation method combined with sucrose density gradients followed by western blot analyses.

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ExosomesSkeletal Muscle FibroblastsDifferential CentrifugationIsolationCharacterizationBiomarkersCell CultureLow Oxygen Conditions

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