Journal
/
/
Isolasjon og karakterisering av immuncellene fra mikro-dissekerte mus Choroid Plexuses
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses
DOI:

09:55 min

February 03, 2022

, , , ,

Chapters

  • 00:05Introduction
  • 00:42Dissection of the Choroid Plexus from the Brain
  • 02:47Preparation of Samples for Flow Cytometry Analysis
  • 03:52Choroid Plexus Myeloid Cells Gating
  • 06:46Choroid Plexus T Cells Gating
  • 07:44Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
  • 09:24Conclusion

Summary

Automatic Translation

Denne studien bruker flowcytometri og to forskjellige gating strategier på isolerte perfused mus hjerne choroid plexuses; Denne protokollen identifiserer de viktigste immuncelledelsettene som fyller denne hjernestrukturen.

Related Videos

Read Article