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Isolation and Characterization of Microvesicles from Peripheral Blood
 

Isolation and Characterization of Microvesicles from Peripheral Blood

Article DOI: 10.3791/55057-v • 06:03 min • January 6th, 2017
January 6th, 2017 •
1, 1, 1, 1, 1
1Department of Hematology/Medical Oncology, University Medical Center Göttingen

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Summary
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Extracellular vesicles present in blood have been suggested as novel biomarkers for various diseases. Here, we present a protocol for the isolation of large plasma membrane-derived microvesicles from peripheral blood samples and their subsequent analysis by conventional flow cytometry and Western Blotting.

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Keywords: Microvesicles Peripheral Blood Plasma Centrifugation Flow Cytometry Characterization Protein Concentration Proinvasive Metastasis Tumor Patients EDTA PBS FCS Antibody
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