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末梢血からの微小胞の単離とキャラクタリゼーション
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation and Characterization of Microvesicles from Peripheral Blood
DOI:

06:03 min

January 06, 2017

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Chapters

  • 00:05Title
  • 00:47Microvesicle (MV) Isolation
  • 02:26MV Flow Cytometric Characterization
  • 03:31Results: Representative Blood-isolated MV Characterization
  • 05:24Conclusion

Summary

Automatic Translation

Extracellular vesicles present in blood have been suggested as novel biomarkers for various diseases. Here, we present a protocol for the isolation of large plasma membrane-derived microvesicles from peripheral blood samples and their subsequent analysis by conventional flow cytometry and Western Blotting.

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