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Isolatie en karakterisering van de immuuncellen van micro-ontleedde muischoroïde plexussen
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses
DOI:

09:55 min

February 03, 2022

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Chapters

  • 00:05Introduction
  • 00:42Dissection of the Choroid Plexus from the Brain
  • 02:47Preparation of Samples for Flow Cytometry Analysis
  • 03:52Choroid Plexus Myeloid Cells Gating
  • 06:46Choroid Plexus T Cells Gating
  • 07:44Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
  • 09:24Conclusion

Summary

Automatic Translation

Deze studie maakt gebruik van flowcytometrie en twee verschillende gating-strategieën op geïsoleerde perfused muizen hersenen choroïde plexussen; dit protocol identificeert de belangrijkste immuuncelsubsets die deze hersenstructuur bevolken.

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