Summary

Erratum: Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published: April 29, 2022

Summary

Abstract

An erratum was issued for: Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice. The Protocol was updated.

Step 6.10 of the Protocol was updated from:

Administer penicillin every 8-12 h after the surgery for 3 days. Monitor the mouse's eating, drinking, moving, and the area operated on. Return the transplant recipient to the company of other animals only after it has fully recovered.
NOTE: The administration of buprenorphine is suggested to prevent post-surgical pain [delete sentence]. The mouse typically recovers from the trauma of the surgery within 3 days. If the mouse is not back to normal feeding and mobility and shows any manifestations of infection, consult a veterinarian for interventions or euthanize it.

to:

Administer buprenorphine subcutaneously at a dose of 0.1 mg/kg body weight every 8 h three times after surgery to alleviate the pain. Monitor the mouse's eating, drinking, moving, and the area operated on. Return the transplant recipient to the company of other animals only after it has fully recovered.
NOTE: The mouse typically recovers from the trauma of the surgery within 3 days. If the mouse is not back to normal feeding and mobility and shows any manifestations of infection, consult a veterinarian for interventions or euthanize it.

Protocol

An erratum was issued for: Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice. The Protocol was updated. Step 6.10 of the Protocol was updated from: Administer penicillin every 8-12 h after the surgery for 3 days. Monitor the mouse's eating, drinking, moving, and the area operated on. Return the transplant recipient to the company of other animals only after it has fully recovered. NOTE: The administration of buprenorphine is suggested to prevent post-surgical pain [delete sentence]. The mouse typically recovers from the trauma of the surgery within 3 days. If the mouse is not back to normal feeding and mobility and shows any manifestations of infection, consult a veterinarian for interventions or euthanize it. to: Administer buprenorphine subcutaneously at a dose of 0.1 mg/kg body weight every 8 h three times after surgery to alleviate the pain. Monitor the mouse's eating, drinking, moving, and the area operated on. Return the transplant recipient to the company of other animals only after it has fully recovered. NOTE: The mouse typically recovers from the trauma of the surgery within 3 days. If the mouse is not back to normal feeding and mobility and shows any manifestations of infection, consult a veterinarian for interventions or euthanize it.

Declarações

The authors have nothing to disclose.

Citar este artigo
Erratum: Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice. J. Vis. Exp. (182), e6498, doi: (2022).

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