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Fabricage van myogene Engineered Tissue constructen
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Biologia
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JoVE Journal Biologia
Fabrication of Myogenic Engineered Tissue Constructs
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Fabricage van myogene Engineered Tissue constructen

DOI:
10.3791/1137-v

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13:43 min

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May 01, 2009

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,
1Department of Anesthesiology,Children’s Hospital Boston and Harvard Medical School, 2Perioperative and Pain Medicine,Children’s Hospital Boston and Harvard Medical School

Capítulos

  • 00:00Título
  • 00:47Introduction
  • 01:23Assembly of Construct Casting Molds
  • 03:25Preparation for Myoblast Cell Isolation
  • 04:56Myoblast Isolation from Skeletal Muscle
  • 09:09Casting Engineered Tissue Constructs
  • 13:17Conclusion

Summary

Tadução automática

Tadução automática

Hier tonen we de fabricage van op basis van collageen, weefsel constructen die het skelet myoblasten. Deze 3-D ontworpen constructies kunnen worden gebruikt om weefsels te vervangen of repareren<em> In vivo</em>. Voor ons doel, hebben we deze als een atrioventriculaire elektrische leiding voor de reparatie van complete hartblok<sup> [1]</sup>.

Tags

FabricationElectronic PacemakersPediatric PatientsCardiac Rhythm DisordersCollagen-based MatrixAutologously-derived CellsRecurrent SurgeriesQuality Of LifePrimary Skeletal Myoblast Cell CulturesHydrogel MatrixElectrical ConduitUpper And Lower Chambers Of The HeartAtrioventricular Electrical ConductionComplete Heart BlockMyoblastsSkeletal MusclesNeonatal Lewis RatsAntibioticsType 1 CollagenMatrigel™NaHCO3

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